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Platforms for Single-Cell Collection and Analysis
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SYSNO ASEP 0508038 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Platforms for Single-Cell Collection and Analysis Author(s) Valihrach, Lukáš (BTO-N) RID, ORCID
Androvič, Peter (BTO-N)
Kubista, Mikael (BTO-N) RIDNumber of authors 3 Article number 807 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 19, č. 3 (2018)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords circulating tumor-cells ; collection ; isolation ; analysis Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA16-10214S GA ČR - Czech Science Foundation (CSF) GA17-04034S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BTO-N - RVO:86652036 UT WOS 000428309800164 EID SCOPUS 85044109576 DOI 10.3390/ijms19030807 Annotation Single-cell analysis has become an established method to study cell heterogeneity and for rare cell characterization. Despite the high cost and technical constraints, applications are increasing every year in all fields of biology. Following the trend, there is a tremendous development of tools for single-cell analysis, especially in the RNA sequencing field. Every improvement increases sensitivity and throughput. Collecting a large amount of data also stimulates the development of new approaches for bioinformatic analysis and interpretation. However, the essential requirement for any analysis is the collection of single cells of high quality. The single-cell isolation must be fast, effective, and gentle to maintain the native expression profiles. Classical methods for single-cell isolation are micromanipulation, microdissection, and fluorescence-activated cell sorting (FACS). In the last decade several new and highly efficient approaches have been developed, which not just supplement but may fully replace the traditional ones. These new techniques are based on microfluidic chips, droplets, micro-well plates, and automatic collection of cells using capillaries, magnets, an electric field, or a punching probe. In this review we summarize the current methods and developments in this field. We discuss the advantages of the different commercially available platforms and their applicability, and also provide remarks on future developments. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2020 Electronic address https://www.mdpi.com/1422-0067/19/3/807/htm
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