Selective elimination of parental chromatin from introgression cultivars of xFestulolium (Festuca × Lolium)

0507497 - ÚEB 2020 RIV CH eng J - Journal Article
Kopecký, David - Horáková, Lucie - Duchoslav, M. - Doležel, Jaroslav
Selective elimination of parental chromatin from introgression cultivars of xFestulolium (Festuca × Lolium).
Sustainability. Roč. 11, č. 11 (2019), č. článku 3153. E-ISSN 2071-1050
R&D Projects: GA ČR(CZ) GA17-13853S; GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Fescue * Genome dominance * Grass * Hybrid * Recombination * Ryegrass
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 2.576, year: 2019
Method of publishing: Open access
http://dx.doi.org/10.3390/su11113153

Alien chromosome introgressions can be used to introduce beneficial traits from one species into another. However, exploitation of the introgressions in breeding requires proper transmission of introgressed segments to consecutive generations. In xFestulolium hybrids chromosomes of Festuca and Lolium readily pair and recombine. This opens a way for introgression of traits (e.g., abiotic and biotic stress resistance) from Festuca into elite Lolium cultivars. However, retention of Festuca chromatin in xFestulolium is uncertain as several studies indicated its gradual elimination over generations of sexual reproduction. Here we investigated genome composition in two subsequent generations of four introgression xFestulolium (F. pratensis × L. multiflorum) cultivars using genomic in situ hybridization. We observed about 27-32% elimination of Festuca chromatin in a single round of multiplication. At this pace, Festuca chromatin would be completely eliminated in about four generations of seed multiplication. On the other hand, we observed that it is possible to increase the proportion of Festuca chromatin in the cultivars by proper selection of mating plants. Nevertheless, once selection is relaxed, the first round of the seed multiplication reverts the genome composition back to the Lolium type. Thus, it seems that amphiploid forms of xFestulolium with relatively stable hybrid genomes may be more promising material for future breeding than introgression lines.
Permanent Link: http://hdl.handle.net/11104/0298477