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Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells
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SYSNO ASEP 0507370 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Effect of iron oxide nanoparticles with ascorbic acid on neural stem cells Author(s) Jiráková, Klára (UEM-P)
Moskvin, Maksym (UMCH-V) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Jendelová, Pavla (UEM-P) RID, ORCIDSource Title Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application. - Ostrava : Tanger Ltd., 2018 - ISBN 9788087294819 Pages s. 668-673 Number of pages 6 s. Publication form Print - P Action International Conference on Nanomaterials - Research and Application (NANOCON) /9./ Event date 18.10.2017 - 20.10.2017 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords neural stem cells ; nanoparticles ; oxidative stress ; ascorbic acid Subject RIV EB - Genetics ; Molecular Biology OECD category Neurosciences (including psychophysiology Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000452823300110 EID SCOPUS 85051847953 Annotation Cells labelled with iron oxide nanoparticles (ION) can be tracked by magnetic resonance imaging (MRI) in several applications. However, various studies demonstrated toxicity and oxidative stress induction associated with nanoparticles exposure. We analysed biologic effects after the exposure of two types of iron oxide nanoparticles (with and without an antioxidative agent, an ascorbic acid) on human neural stem cells. The labelled cells in gel phantoms were detected in MRI and they showed decreased relaxation rates in comparison with control. ION slightly decreased cell proliferation in comparison with unlabelled cells, which was dependent on concentration and presence of ascorbic acid. None of the nanoparticle type showed negative effect on cell viability and both demonstrated minor effect on reactive oxygen species (ROS) formation. Unfortunately, ascorbic acid bound to nanoparticles did not show any effect on ROS attenuation. Cells exposed to both types of nanoparticles showed increased positivity for a phosphorylated form of H2AX a marker of double strand breaks. We showed that ION in low concentrations do not affect cell viability, but have negative effect on cells on DNA level. Their potential use for oxidative stress reduction is dependent on the concentration of ascorbic acid bound to the nanoparticles and this should be further increased. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2020
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