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DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES
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SYSNO ASEP 0506727 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title DNA POLYMERASE STOP ASSAY FOR DETECTION OF G-QUADRUPLEXES Author(s) Petr, Marek (BFU-R) ORCID
Bažantová, Pavla (BFU-R)
Adámik, Matěj (BFU-R) ORCID
Kejnovská, Iva (BFU-R) RID, ORCID
Dvořáková, Zuzana (BFU-R) ORCID
Vorlíčková, Michaela (BFU-R) RID, ORCID
Pečinka, Petr (BFU-R) RID
Brázdová, Marie (BFU-R) RID, ORCIDNumber of authors 8 Source Title CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. - Brno : Institute of Analytical Chemistry AS CR, 2014 / Foret F. ; Křenková J. ; Drobníková I. ; Guttman A. ; Klepárník K. - ISBN 978-80-904959-2-0 Pages č. 2014 (2014), s. 349-353 Number of pages 5 s. Publication form Print - P Action CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./ Event date 20.10.2014-22.10.2014 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords DNA Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Institutional support BFU-R - RVO:68081707 UT WOS 000354547400114 Annotation In this work we performed DNA polymerase stop assays while using template DNA derived from promoter regions of VEGF and c-Myc proto-oncogenes under variety of experimental conditions. Partial stopping of DNA synthesis along the template strand was observed in the presence of K+ ions due to formation of stable G-quadruplex structures. In contrast, Na+ ions alone were unable to stabilize G-quadruplexes to stop the reaction at their site. These data suggest that K+ and Na+ ions, which are both known to stabilize G-quadruplexes, do this in different manner or extent. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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