Oligopeptide-targeted polymer nanoprobes for fluorescence-guided endoscopic surgery

Pola, Robert; Parnica, Jozef; Zuska, Kryštof; Böhmová, Eliška; Filipová, Marcela; Pechar, Michal; Pankrác, J.; Mucksová, J.; Kalina, J.; Trefil, P.; Šefc, L.; Větvička, D.; Poučková, P.; Bouček, J.; Janoušková, Olga; Etrych, Tomáš

doi:https://dx.doi.org/10.1088/2399-7532/ab159e

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Oligopeptide-targeted polymer nanoprobes for fluorescence-guided endoscopic surgery

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SYSNO ASEP	0506036
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Oligopeptide-targeted polymer nanoprobes for fluorescence-guided endoscopic surgery
Author(s)	Pola, Robert (UMCH-V)_{RID, ORCID} Parnica, Jozef (UMCH-V) Zuska, Kryštof (UMCH-V) Böhmová, Eliška (UMCH-V)_RID Filipová, Marcela (UMCH-V)_{RID, ORCID} Pechar, Michal (UMCH-V)_{RID, ORCID} Pankrác, J. (CZ) Mucksová, J. (CZ) Kalina, J. (CZ) Trefil, P. (CZ) Šefc, L. (CZ) Větvička, D. (CZ) Poučková, P. (CZ) Bouček, J. (CZ) Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Etrych, Tomáš (UMCH-V)_{RID, ORCID}
Article number	024004
Source Title	Multifunctional Materials. - : Institute of Physics Publishing - ISSN 2399-7532 Roč. 2, č. 2 (2019), s. 1-13
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	polymer carrier ; endoscopic surgery ; peptide targeting
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	NV16-28594A GA MZd - Ministry of Health (MZ)
	FV10370 GA MPO - Ministry of Industry and Trade (MPO)
	GA16-17207S GA ČR - Czech Science Foundation (CSF)
	GA17-13283S GA ČR - Czech Science Foundation (CSF)
	LO1507 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
DOI	10.1088/2399-7532/ab159e
Annotation	A polymer probe based on N-(2-hydroxypropyl)methacrylamide copolymers labelled with a fluorescent dye Dy-633 or Cy-7 and decorated with targeting oligopeptides GE-7 or GE-11, specific targeting ligands binding to epidermal growth factor receptor (EGFR) highly expressed on surface of tumour cells, was designed, synthesised and characterised. Specific accumulation of the polymer probe in the tumour mass is a prerequisite for successful fluorescence-guided endoscopic surgery as the fluorescence signal from the malignant cells enables more precise resection of the tumour without damaging the healthy tissue. Flow cytometry and confocal microscopy was used to assess the binding efficacy of the oligopeptide conjugates to EGFR on the cell membranes of the malignant cells. The results showed that the highest binding efficacy was achieved with polymers bearing the GE-11 targeting oligopeptide in human EGFR-positive hypopharyngeal carcinoma cells (FaDu) and in breast adenocarcinoma cells (MDA-MB-231). Similarly, the polymer probes targeted by the GE-11 oligopeptidewere found in vivo as highly effective in tumour accumulation, as determined from fluorescence imaging. Indeed, the ex vivo cross-section of the tumours showed significant tumour border fluorescence proving the potential of the studied polymer probes. Moreover, the presence of the active targeting moiety on the polymer-drug conjugate should enable the use of such a conjugate as a targeted polymer system for treatment of solid tumours. Replacement of the fluorescent probe with a cytostatic drug provides a targeted polymer nanocancerostatic for advanced treatment of neoplastic diseases, thus the polymer probes have multiple functions.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2020
Electronic address	https://iopscience.iop.org/article/10.1088/2399-7532/ab159e

Number of the records: 1