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Cellular response to proton radiation
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SYSNO ASEP 0504674 Document Type D - Thesis R&D Document Type The record was not marked in the RIV Title Cellular response to proton radiation Author(s) Jelínek Michaelidesová, Anna (UJF-V) RID, ORCID, SAI Number of authors 1 Issue data Praha: CTU, 2018 Number of pages 109 s. Publication form Print - P Language eng - English Country CZ - Czech Republic Keywords proton therapy ; relative biological effectiveness ; bystander effect ; scattered beam ; pencil beam Subject RIV FP - Other Medical Disciplines OECD category Radiology, nuclear medicine and medical imaging R&D Projects GBP108/12/G108 GA ČR - Czech Science Foundation (CSF) Institutional support UJF-V - RVO:61389005 Annotation The aim of the dissertation was to examine several aspects of the biological effectiveness of therapeutic proton beams. From the in-vitro study at two proton facilities using 30 MeV proton beams and three biological endpoints (cell survival, percentage of binuclear cells containing micronuclei, and micronuclei frequencies) for normal human neonatal dermal fibroblasts, relative biological effctiveness values of 1.36+-0.21 for the scanning beam and 1.29 +- 0.05 for the broad beam at 50% survival level compared to a 60Co source were found. The percentage of binuclear cells containing micronuclei express a linear behavior with increasing dose for both proton beams and gamma-rays as well. The micronuclei frequencies express a linear-quadratic behavior with increasing dose for the three different beams. Examinating the bystander effect using the medium transfer method, normal human neonatal dermal fibroblasts and cell survival as the biological endpoint, no significant differences between protons and photons were found. Investigating the relative biological effectiveness at several positions of a Spread-out Bragg peak formed by a clinical pencil proton beam, normal human neonatal dermal fibroblasts, cell survival and micronuclei frequencies as biological endpoints, relative biological effectiveness values at the distal position of Spread-out Bragg peak were estimated to be 2.05 +- 0.44, 1.85 +- 0.42, 1.53 +- 0.38 for survival levels 90, 50 and 10%, respectively. An increasing complexity of chromosomal DNA damage was observed from the micronuclei assay towards the distal end of the Spread-out Bragg peak, where the linear energy transfer values are higher. Similar observations were obtained using fractionated irradiation schemes, fibroblasts AG01522 and cell survival as a biological endpoint. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2020
Number of the records: 1