Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

Ježek, Jan; Pilát, Zdeněk; Bernatová, Silvie; Kirchhoff, F.; Tannert, A.; Neugebauer, U.; Samek, Ota; Zemánek, Pavel

doi:https://dx.doi.org/10.1117/12.2517933

Number of the records: 1

Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips

1.

SYSNO ASEP	0498740
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Laser tweezers Raman spectroscopy of E. coli under antibiotic stress in microfluidic chips
Author(s)	Ježek, Jan (UPT-D)_{RID, ORCID, SAI} Pilát, Zdeněk (UPT-D)_{RID, SAI, ORCID} Bernatová, Silvie (UPT-D)_{RID, SAI} Kirchhoff, F. (DE) Tannert, A. (DE) Neugebauer, U. (DE) Samek, Ota (UPT-D)_{RID, ORCID, SAI} Zemánek, Pavel (UPT-D)_{RID, SAI, ORCID}
Number of authors	8
Article number	1097618
Source Title	21st Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics. (Proceedings of SPIE 10976). - Bellingham : SPIE, 2018 / Zemánek Pavel - ISSN 0277-786X - ISBN 9781510626072
Number of pages	8 s.
Publication form	Print - P
Action	Czech-Polish-Slovak Optical Conference on Wave and Quantum Aspects of Contemporary Optics /21./
Event date	03.09.2018 - 07.09.2018
VEvent location	Lednice
Country	CZ - Czech Republic
Event type	EUR
Language	eng - English
Country	US - United States
Keywords	Raman microspectroscopy ; optical tweezers ; optofluidics ; E. coli - antibiotics
Subject RIV	BH - Optics, Masers, Lasers
OECD category	Optics (including laser optics and quantum optics)
R&D Projects	GA16-07965S GA ČR - Czech Science Foundation (CSF)
	LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UPT-D - RVO:68081731
UT WOS	000455324600043
EID SCOPUS	85059390432
DOI	10.1117/12.2517933
Annotation	Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Because of the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We used laser tweezers Raman spectroscopy (LTRS) in a microchamber chip to manipulate and analyze individual E. coli cells. We subjected the cells to antibiotic cefotaxime, and we observed the changes by the time-lapse microscopy and Raman spectroscopy. We found observable changes in the cellular morphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.
Workplace	Institute of Scientific Instruments
Contact	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Year of Publishing	2019

Number of the records: 1