Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

Olšinová, Marie; Jurkiewicz, Piotr; Kishko, Irina; Sýkora, Jan; Sabó, Ján; Hof, Martin; Cwiklik, Lukasz; Cebecauer, Marek

doi:https://dx.doi.org/10.1016/j.isci.2018.11.026

Number of the records: 1

Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

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SYSNO ASEP	0497636
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics
Author(s)	Olšinová, Marie (UFCH-W)_RID Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Kishko, Irina (UFCH-W) Sýkora, Jan (UFCH-W)_RID Sabó, Ján (UFCH-W) Hof, Martin (UFCH-W)_{RID, ORCID} Cwiklik, Lukasz (UFCH-W)_{RID, ORCID} Cebecauer, Marek (UFCH-W)_{RID, ORCID, SAI}
Source Title	iScience. - : Cell Press Roč. 10, DEC 2018 (2018), s. 87-97
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	LATERAL DIFFUSION ; HYDROPHOBIC MISMATCH ; PROTEIN INTERACTIONS
Subject RIV	BO - Biophysics
OECD category	Biophysics
R&D Projects	GAP305/11/0459 GA ČR - Czech Science Foundation (CSF)
	GBP208/12/G016 GA ČR - Czech Science Foundation (CSF)
	GA18-26751S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	UFCH-W - RVO:61388955
UT WOS	000454331700008
DOI	10.1016/j.isci.2018.11.026
Annotation	The dynamics of cellular membranes is primarily determined by lipid species forming a bilayer. Proteins are considered mainly as effector molecules of diverse cellular processes. In addition to large assemblies of proteins, which were found to influence properties of fluid membranes, biological membranes are densely populated by small, highly mobile proteins. However, little is known about the effect of such proteins on the dynamics of membranes. Using synthetic peptides, we demonstrate that transmembrane helices interfere with the mobility of membrane components by trapping lipid acyl chains on their rough surfaces. The effect is more pronounced in the presence of cholesterol, which segregates from the rough surface of helical peptides. This may contribute to the formation or stabilization of membrane heterogeneities. Since roughness is a general property of helical transmembrane segments, our results suggest that, independent of their size or cytoskeleton linkage, integral membrane proteins affect local membrane dynamics and organization.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2019

Number of the records: 1