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Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots
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SYSNO ASEP 0497527 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots Author(s) Dvořák, Miloš (UIACH-O) ORCID, RID
Miková, B. (CZ)
Šlampová, Andrea (UIACH-O) RID
Kubáň, Pavel (UIACH-O) RID, ORCIDNumber of authors 4 Source Title CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. - Brno : Ústav analytické chemie AV ČR, v. v. i., 2018 / Foret F. ; Křenková J. ; Drobníková I. ; Klepárník K. ; Přikryl J. - ISBN 978-80-904959-5-1 Pages s. 52-54 Number of pages 3 s. Publication form Online - E Action CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./ Event date 15.10.2018 - 17.10.2018 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords hollow fibre-liquid phase microextraction ; microextraction of basic drugs ; dried blood spots Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA18-13135S GA ČR - Czech Science Foundation (CSF) GA16-09135S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2019 Electronic address http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Number of the records: 1