Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors

0494959 - ÚEM 2019 RIV GB eng J - Journal Article
Mukherjee, S.P. - Bondarenko, O. - Kohonen, P. - Andon, F.T. - Brzicová, Táňa - Gessner, A. - Mathur, S. - Bottini, M. - Calligari, P. - Stella, L. - Kisin, E. - Shvedová, A. - Autio, R. - Salminen-Mankonen, H. - Lahesmaa, R. - Fadeel, B.
Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors.
Scientific Reports. Roč. 8, jan (2018), č. článku 1115. ISSN 2045-2322. E-ISSN 2045-2322
Institutional support: RVO:68378041
Keywords : pattern-recognition receptors * graphene oxide * protein corona * immune-system
OECD category: Cell biology
Impact factor: 4.011, year: 2018

Carbon-based nanomaterials including carbon nanotubes (CNTs) have been shown to trigger inflammation. However, how these materials are 'sensed' by immune cells is not known. Here we compared the effects of two carbon-based nanomaterials, single-walled CNTs (SWCNTs) and graphene oxide (GO), on primary human monocyte-derived macrophages. Genome-wide transcriptomics assessment was performed at sub-cytotoxic doses. Pathway analysis of the microarray data revealed pronounced effects on chemokine-encoding genes in macrophages exposed to SWCNTs, but not in response to GO, and these results were validated by multiplex array-based cytokine and chemokine profiling. Conditioned medium from SWCNT-exposed cells acted as a chemoattractant for dendritic cells. Chemokine secretion was reduced upon inhibition of NF-kappa B, as predicted by upstream regulator analysis of the transcriptomics data, and Toll-like receptors (TLRs) and their adaptor molecule, MyD88 were shown to be important for CCL5 secretion. Moreover, a specific role for TLR2/4 was confirmed by using reporter cell lines. Computational studies to elucidate how SWCNTs may interact with TLR4 in the absence of a protein corona suggested that binding is guided mainly by hydrophobic interactions. Taken together, these results imply that CNTs may be 'sensed' as pathogens by immune cells.
Permanent Link: http://hdl.handle.net/11104/0288014