Number of the records: 1
Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level during ART
- 1.
SYSNO ASEP 0494673 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Expression of TIM-3 on Plasmacytoid Dendritic Cells as a Predictive Biomarker of Decline in HIV-1 RNA Level during ART Author(s) Font-Haro, Albert (UMG-J)
Janovec, Vaclav (UMG-J)
Hofman, T. (CZ)
Machala, L. (CZ)
Jilich, D. (CZ)
Mělková, Z. (CZ)
Weber, J. (CZ)
Trejbalová, Kateřina (UMG-J) RID
Hirsch, Ivan (UMG-J) ORCID, RIDNumber of authors 9 Article number 154 Source Title Viruses. - : MDPI
Roč. 10, č. 4 (2018)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords hiv-1 ; antiretroviral therapy (ART) ; innate and adaptive immune responses ; plasmacytoid dendritic cells (pDCs) ; pDC dysfunction ; T cell Ig and mucin-domain containing molecule 3 (TIM-3) ; bdca-2 ; Toll-like receptors 7 and 9 (TLR7/9) Subject RIV EB - Genetics ; Molecular Biology OECD category Virology R&D Projects GA14-32547S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000435184400015 DOI 10.3390/v10040154 Annotation Depletion and functional impairment of circulating plasmacytoid dendritic cells (pDCs) are characteristic attributes of HIV-1-infection. The mechanism of dysfunction of pDCs is unclear. Here, we studied the development of phenotype of pDCs in a cohort of HIV-1-infected individuals monitored before the initiation and during a 9-month follow up with antiretroviral therapy (ART). Using polychromatic flow cytometry, we detected significantly higher pDC-surface expression of the HIV-1 receptor CD4, regulatory receptor BDCA-2, Fcy receptor CD32, pDC dysfunction marker TIM-3, and the marker of killer pDC, TRAIL, in treatment-naive HIV-1-infected individuals before initiation of ART when compared to healthy donors. After 9 months of ART, all of these markers approached but did not reach the expression levels observed in healthy donors. We found that the rate of decline in HIV-1 RNA level over the first 3 months of ART negatively correlated with the expression of TITM-3 on pDCs. We conclude that immunogenic phenotype of pDCs is not significantly restored after sustained suppression of HIV-1 RNA level in ART-treated patients and that the level of the TIM-3 expressed on pDCs in treatment naive patients could be a predictive marker of the rate of decline in the HIV-1 RNA level during ART. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2019
Number of the records: 1