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Encyclopedia of Biophysics
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SYSNO ASEP 0494619 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Fluorescence Correlation Spectroscopy of Lipid Membranes Author(s) Macháň, Radek (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Encyclopedia of Biophysics. - Geneve : Springer Nature Switzerland AG, 2018 - ISBN 978-3-642-35943-9 Pages s. 1-8 Number of pages 34 s. Number of pages 1000 Publication form Online - E Language eng - English Country CH - Switzerland Keywords fluorescence ; spectroscopy ; lipid membranes Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry Institutional support UFCH-W - RVO:61388955 DOI 10.1007/978-3-642-35943-9_560-1 Annotation Fluorescence correlation spectroscopy (FCS), along with fluorescence recovery after photobleaching (FRAP) and single particle tracking (SPT), belongs among the most important techniques for investigating molecular diffusion in biological systems, including lipid membranes. FCS extracts information on the kinetics of molecular movements and chemical reactions from the analysis of fluctuations in fluorescence intensity collected from a small observation volume. When applied to study mobility of molecules in biological membranes and their artificial models, it has to be adapted to the slow mobility of membrane components and the locally planar geometry. In biological membranes, their highly inhomogeneous structure ought to be considered as well. According to current models, cell membranes contain nanoscopic domains differing in composition and mechanical properties from their surroundings. Furthermore, interaction with cytoskeleton forms semipermeable barriers to membrane... Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2019 Electronic address https://link.springer.com/referenceworkentry/10.1007/978-3-642-35943-9_560-1
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