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Capillary Electromigration Separation Methods
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SYSNO ASEP 0492951 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Application of capillary electromigration methods for physicochemical measurements Author(s) Štěpánová, Sille (UOCHB-X) ORCID
Kašička, Václav (UOCHB-X) RID, ORCIDSource Title Capillary Electromigration Separation Methods. - Amsterdam : Elsevier, 2018 / Poole Colin F. - ISBN 978-0-12-809375-7 Pages s. 547-591 Number of pages 45 s. Number of pages 626 Publication form Print - P Language eng - English Country NL - Netherlands Keywords acidity constant ; binding constant ; capillary electrophoresis ; effective charge ; electrophoretic mobility Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA15-01948S GA ČR - Czech Science Foundation (CSF) GA17-10832S GA ČR - Czech Science Foundation (CSF) Institutional support UOCHB-X - RVO:61388963 UT WOS 000462135300022 DOI 10.1016/B978-0-12-809375-7.00024-1 Annotation In this chapter, high-performance capillary electromigration (HPCE or CE) methods are presented as powerful tools for the determination of important physicochemical parameters of (bio)molecules. Using the different HPCE methods (zone electrophoresis in free solutions or in sieving/gel media, isotachophoresis, isoelectric focusing, affinity electrophoresis, and electrokinetic chromatography), the following physicochemical characteristics of a wide spectrum of compounds can be determined: effective, ionic and limiting mobilities, effective charges, isoelectric points, Stokes radii, relative molecular masses, partition constants, diffusion coefficients, and acidity (ionization) constants (pKa). In addition, the binding (stability, association, formation, or dissociation) constants of (bio)molecular complexes and rate constants of chemical and enzymatic reactions and noncovalent molecular interactions can be estimated. Advantages of HPCE methods for these physicochemical measurements include high separation efficiency, short analysis time, and especially the ultra-small mass (picogram range) and volume (nanoliter level) of injected solute samples and low consumption of chemicals and solvents. Moreover, the characterization of not quite pure solutes and solutes sparingly soluble in water is possible. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2019
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