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p73, like its p53 homolog, shows preference for inverted repeats forming cruciforms
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SYSNO ASEP 0492335 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title p73, like its p53 homolog, shows preference for inverted repeats forming cruciforms Author(s) Čechová, Jana (BFU-R)
Coufal, Jan (BFU-R) ORCID
Jagelská, Eva (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Brázda, Václav (BFU-R) RID, ORCIDNumber of authors 5 Article number e0195835 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 13, č. 4 (2018)Number of pages 13 s. Language eng - English Country US - United States Keywords sequence-specific binding ; dna-binding ; transcriptional activity ; supercoiled dna Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects GA15-21855S GA ČR - Czech Science Foundation (CSF) EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 UT WOS 000430290200060 DOI 10.1371/journal.pone.0195835 Annotation p73 is a member of the p53 protein family and has essential functions in several signaling pathways involved in development, differentiation, DNA damage responses and cancer. As a transcription factor, p73 achieves these functions by binding to consensus DNA sequences and p73 shares at least partial target DNA binding sequence specificity with p53. Transcriptional activation by p73 has been demonstrated for more than fifty p53 targets in yeast and/or human cancer cell lines. It has also been shown previously that p53 binding to DNA is strongly dependent on DNA topology and the presence of inverted repeats that can form DNA cruciforms, but whether p73 transcriptional activity has similar dependence has not been investigated. Therefore, we evaluated p73 binding to a set of p53-response elements with identical theoretical binding affinity in their linear state, but different probabilities to form extra helical structures. We show by a yeast-based assay that transactivation in vivo correlated more with the relative propensity of a response element to form cruciforms than to its expected in vitro DNA binding affinity. Structural features of p73 target sites are therefore likely to be an important determinant of its transactivation function. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2019
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