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Silica monolithic capillary columns for hilic separations
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SYSNO ASEP 0492059 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Silica monolithic capillary columns for hilic separations Author(s) Moravcová, Dana (UIACH-O) RID, ORCID
Planeta, Josef (UIACH-O) RID, ORCIDNumber of authors 2 Source Title Hungarian Journal of Industrial Chemistry. - : University of Veszprem - ISSN 0133-0276
Roč. 46, č. 1 (2018), s. 39-42Number of pages 4 s. Publication form Print - P Language eng - English Country HU - Hungary Keywords silica monolithic columns ; nucleotide ; capillary chromatography Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects VI20172020069 GA MV - Ministry of Interior (MV) GA16-03749S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000440079700009 DOI 10.1515/hjic-2018-0009 Annotation The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2-methacryloyloxyethylphosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interest. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2019
Number of the records: 1