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Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil
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SYSNO ASEP 0491770 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil Author(s) Slavíčková, Michaela (UOCHB-X) RID
Janoušková, Martina (MBU-M) RID, ORCID
Šimonová, Anna (UOCHB-X)
Cahová, Hana (UOCHB-X) RID, ORCID
Kambová, Milada (MBU-M)
Šanderová, Hana (MBU-M) RID, ORCID
Krásný, Libor (MBU-M) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCIDSource Title Chemistry - A European Journal. - : Wiley - ISSN 0947-6539
Roč. 24, č. 33 (2018), s. 8311-8314Number of pages 4 s. Language eng - English Country DE - Germany Keywords DNA ; epigenetics ; nucleotides ; RNA polymerase ; transcription Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA17-03419S GA ČR - Czech Science Foundation (CSF) Institutional support UOCHB-X - RVO:61388963 ; MBU-M - RVO:61388971 UT WOS 000435269000012 EID SCOPUS 85048638047 DOI 10.1002/chem.201801757 Annotation Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5-ethynyluracil (U-E) with azides was used for turning off sequence-specific protein-DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI-HF. Finally, DNA template containing U-E was used for in vitro transcription with E.coli RNA polymerase and the transcription was turned off by CuAAC with 3-azidopropane-1,2-diol or 3-azido-7-hydroxycoumarin. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2019
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