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Capillary Electromigration Separation Methods
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SYSNO ASEP 0491723 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Capillary Gel and Sieving Electrophoresis Author(s) Mikšík, Ivan (FGU-C) RID, ORCID, SAI Source Title Capillary Electromigration Separation Methods. - Amsterdam : Elsevier, 2018 / Poole Colin F. - ISBN 978-0-12-809375-7 Pages s. 143-165 Number of pages 23 s. Number of pages 626 Publication form Print - P Language eng - English Country NL - Netherlands Keywords capillary gel electrophoresis ; nucleic acid analysis ; DNA analysis ; protein analysis ; lab-on-a-chip ; miniaturization Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA17-10832S GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000462135300006 DOI 10.1016/B978-0-12-809375-7.00006-X Annotation Capillary gel electrophoresis (CGE) is a powerful method for analysis of biopolymers such as DNA, RNA, and proteins by sieving phenomena, according to size (molecular weight). This technique evolved from “traditional” gel electrophoresis but has many advantages, such as automation, high separation efficiency, on-capillary detection, anticonvective nature of the capillary, and array dimension (multicapillary system). An interesting improvement is also miniaturization, lab-on-a-chip should also be emphasized, including two-dimensional separation. All these properties and technical properties are reviewed. Special attention is paid to gels used (polymers), their preparation, and applicability. Particular parts of the chapter are examples of applications of CGE for analysis of real samples. Commercially available resources, such as instruments and kits, are also reviewed. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
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