Analýza významných biologických látek pomocí luminiscenčních senzorů

0490775 - ÚIACH 2019 CZ eng D - Thesis
Datinská, Vladimíra
Analýza významných biologických látek pomocí luminiscenčních senzorů.
[Analysis of biologically relevant substances by luminescence sensors.]
Ústav analytické chemie AV ČR, v. v. i. Defended: Brno. 22.06.2018. - Brno: Masarykova univerzita, 2018. xv, 77
Institutional support: RVO:68081715
Keywords : bioconjugation * capillary electrophoresis * Förster resonance energy transfer * quantum dots
OECD category: Analytical chemistry

Bioanalysis is recently developing and progressing area, which generally uses luminescent labels in order to increase the sensitivity. This work is focused on quantum dots nanocrystals as luminophores. Quantum dots were conjugated with biologically relevant molecules (proteins and nucleic acids) and conjugates were characterized by electromigration methods. A quantum dot probe is based on Förster resonance energy transfer and it is intended for detection of a specific nucleic acid sequence. Furthermore, isotachophoresis is presented as an alternative way of nucleic acids sample preparation. Finally, an advanced synthetic method of immunoprobe based on quantum dot is described.
Permanent Link: http://hdl.handle.net/11104/0284920