Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer

0489805 - ÚIACH 2019 RIV DE eng J - Journal Article
Datinská, Vladimíra - Klepárník, Karel - Belšánová, B. - Minárik, M. - Foret, František
Capillary electrophoresis, a method for the determination of nucleic acid ligands covalently attached to quantum dots representing a donor of Forster resonance energy transfer.
Journal of Separation Science. Roč. 41, č. 14 (2018), s. 2961-2968. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA TA ČR(CZ) TA02010672; GA ČR(CZ) GA17-01995S
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * Förster resonance energy transfer * nucleic acids * quantum dots
OECD category: Analytical chemistry
Impact factor: 2.516, year: 2018

The synthesis and determination of the structure of a Förster resonance energy transfer probe intended for the detection of specific nucleic acid sequences are described here. The probe is based on the hybridization of oligonucleotide modified quantum dots with a fluorescently labeled nucleic acid sample resulting in changes of the fluorescence emission due to the energy transfer effect.
Permanent Link: http://hdl.handle.net/11104/0284149