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Localization of RNA and translation in the mammalian oocyte and embryo
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SYSNO ASEP 0489565 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Localization of RNA and translation in the mammalian oocyte and embryo Author(s) Jansová, Denisa (UZFG-Y) ORCID
Tětková, Anna (UZFG-Y) ORCID
Končická, Markéta (UZFG-Y) ORCID
Kubelka, Michal (UZFG-Y) RID, ORCID
Šušor, Andrej (UZFG-Y) RID, ORCIDArticle number e0192544 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 13, č. 3 (2018)Number of pages 15 s. Publication form Online - E Language eng - English Country US - United States Keywords RNA localization ; mammalian oocyte Subject RIV EB - Genetics ; Molecular Biology OECD category Biochemistry and molecular biology R&D Projects GA13-12291S GA ČR - Czech Science Foundation (CSF) GA15-22765S GA ČR - Czech Science Foundation (CSF) EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UZFG-Y - RVO:67985904 UT WOS 000427189300005 EID SCOPUS 85043777411 DOI 10.1371/journal.pone.0192544 Annotation The tight correlation between mRNA distribution and subsequent protein localization and function indicate a major role for mRNA localization within the cell. RNA localization, followed by local translation, presents a mechanism for spatial and temporal gene expression regulation utilized by various cell types. However, little is known about mRNA localization and translation in the mammalian oocyte and early embryo. Importantly, fully-grown oocyte becomes transcriptionally inactive and only utilizes transcripts previously synthesized and stored during earlier development. We discovered an abundant RNA population in the oocyte and early embryo nucleus together with RNA binding proteins. We also characterized specific ribosomal proteins, which contribute to translation in the oocyte and embryo. By applying selected markers to mouse and human oocytes, we found that there might be a similar mechanism of RNA metabolism in both species. In conclusion, we visualized the localization of RNAs and translation machinery in the oocyte, that could shed light on this terra incognita of these unique cell types in mouse and human. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2019
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