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Coping with iron limitation: a metabolomic study of Synechocystis sp PCC 6803
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SYSNO ASEP 0489000 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Coping with iron limitation: a metabolomic study of Synechocystis sp PCC 6803 Author(s) Rivas-Ubach, A. (ES)
Poret-Peterson, A. T. (US)
Penuelas, J. (ES)
Sardans, J. (ES)
Pérez-Trujillo, M. (ES)
Legido-Quigley, C. (GB)
Oravec, Michal (UEK-B) RID, ORCID, SAI
Urban, Otmar (UEK-B) RID, ORCID, SAI
Elser, J. J. (US)Number of authors 9 Article number 28 Source Title Acta Physiologiae Plantarum. - : Springer - ISSN 0137-5881
Roč. 40, č. 2 (2018)Number of pages 13 s. Language eng - English Country PL - Poland Keywords sp strain pcc-6803 ; ocean acidification ; unicellular cyanobacterium ; marine-phytoplankton ; foliar metabolomes ; nitrogen-fixation ; metal homeostasis ; oxidative stress ; pacific-ocean ; responses ; Metabolomics ; Metallomics ; Iron limitation ; Cyanobacteria ; Ecological stoichiometry Subject RIV EH - Ecology, Behaviour OECD category Environmental sciences (social aspects to be 5.7) R&D Projects LO1415 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015061 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEK-B - RVO:86652079 UT WOS 000424497300007 EID SCOPUS 85044352921 DOI 10.1007/s11738-018-2603-1 Annotation Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N-2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA: DNA, C, N, and P, and higher ratios of protein: RNA, C: N, C: P, and N: P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C: P and N: P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2019
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