Number of the records: 1
Exocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation
- 1.0488738 - ÚEB 2019 RIV US eng J - Journal Article
Kulich, I. - Vojtíková, Z. - Sabol, P. - Ortmannová, Jitka - Neděla, Vilém - Tihlaříková, Eva - Žárský, Viktor
Exocyst subunit EXO70H4 has a specific role in callose synthase secretion and silica accumulation.
Plant Physiology. Roč. 176, č. 3 (2018), s. 2040-2051. ISSN 0032-0889. E-ISSN 1532-2548
Institutional support: RVO:61389030 ; RVO:68081731
Keywords : COMPLETE PENETRATION RESISTANCE * SCANNING-ELECTRON-MICROSCOPE * POWDERY MILDEW
OECD category: Cell biology; Plant sciences, botany (UPT-D)
Impact factor: 6.305, year: 2018
Biogenesis of the plant secondary cell wall involves many important aspects, such as phenolic compound deposition and often silica encrustation. Previously, we demonstrated the importance of the exocyst subunit EXO70H4 for biogenesis of the trichome secondary cell wall, namely for deposition of the autofluorescent and callose-rich cell wall layer. Here, we reveal that EXO70H4-driven cell wall biogenesis is cons titutively active in the mature trichome, but also can be activated elsewhere upon pathogen attack, giving this study a broader significance with an overlap into phytopathology. To address the specificity of EXO70H4 among the EXO70 family, we complemented the exo70H4-1 mutant by 18 different Arabidopsis (Arabidopsis thaliana) EXO70 paralogs subcloned under the EXO70H4 promoter. Only EXO70H4 had the capacity to rescue the exo70H4-1 trichome phenotype. Callose deposition phenotype of exo70H4-1 mutant is caused by impaired secretion of PMR4, a callose synthase responsible for the synthesis of callose in the trichome. PMR4 colocalizes with EXO70H4 on plasma membrane microdomains that do not develop in the exo70H4-1 mutant. Using energy-dispersive x-ray microanalysis, we show that both EXO70H4- and PMR4-dependent callose deposition in the trichome are essential for cell wall silicification.
Permanent Link: http://hdl.handle.net/11104/0283280
File Download Size Commentary Version Access 2018_Kulich_PLANT PHYSIOLOGY_2040.pdf 12 2.3 MB Other open-access
Number of the records: 1