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Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method
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SYSNO ASEP 0488709 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method Author(s) Kvach, Yuriy (UBO-W) ORCID, RID, SAI
Ondračková, Markéta (UBO-W) RID, ORCID, SAI
Janáč, Michal (UBO-W) RID, ORCID, SAI
Jurajda, Pavel (UBO-W) RID, SAI, ORCIDNumber of authors 4 Source Title Diseases of Aquatic Organisms. - : Inter-Research - ISSN 0177-5103
Roč. 127, č. 3 (2018), s. 213-224Number of pages 12 s. Language eng - English Country DE - Germany Keywords flounder Paralichthys-olivaceus ; Neoheterobothrium-hirame ; community structure ; infection levels ; Baltic sea ; Odontobutidae ; ectoparasites ; Perciformes ; collection ; ecology ; Parasite community ; Preservation methods ; Perca fluviatilis ; Rhodeus amarus ; Methodology ; Parasitological examination Subject RIV GL - Fishing OECD category Fishery R&D Projects GBP505/12/G112 GA ČR - Czech Science Foundation (CSF) Institutional support UBO-W - RVO:68081766 UT WOS 000427021400005 EID SCOPUS 85043698364 DOI https://doi.org/10.3354/dao03197 Annotation The aim of this study was to evaluate the influence of preservation method on the results of parasite community studies. Two host species, European perch Perca fluviatilis and European bitterling Rhodeus amarus, were examined for parasites after having been subjected to 4 different storage treatments: freezing, preservation in 4% formaldehyde or 70% ethanol and transportation of live (fresh) fish as a control. Preservation prior to dissection resulted in a loss of information, leading to incomplete quantitative data (all preservation treatments), qualitative data (ethanol and formaldehyde preservation) and a lowered ability to determine parasites to species level based on morphology compared to dissecting fresh fish. Of the more abundant taxa, only crustaceans and acanthocephalans provided relatively even results between treatments. We conclude that preservation media, such as ethanol or formaldehyde, significantly affects the ability to obtain precise parasite community data, hence, we recommend the use of freshly sacrificed fish for parasite community studies whenever possible. Alternatively, freezing may prove acceptable for evaluating parasite community taxonomic composition. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2019
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