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Protein-protein interactions in the sperm membrane prior to fusion with the egg.
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SYSNO ASEP 0482838 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Protein-protein interactions in the sperm membrane prior to fusion with the egg. Author(s) Dvořáková-Hortová, Kateřina (BTO-N)
Děd, Lukáš (BTO-N) RID
Šebková, Nataša (BTO-N)
Frolíková, Michaela (BTO-N) ORCIDNumber of authors 4 Source Title Program and Abstract Book of the 4th World Congress of Reproductive Biology. - Okinawa : Society for Reproduction and Development, 2017 Action World Congress of Reproductive Biology 2017 Event date 27.09.2017 - 29.09.2017 VEvent location Okinawa Country JP - Japan Language eng - English Country JP - Japan Keywords acrosome reaction ; CD46 ; STED Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA14-05547S GA ČR - Czech Science Foundation (CSF) Institutional support BTO-N - RVO:86652036 Annotation The acrosome reaction (AR) is a process of membrane fusion and lytic enzyme release, which enables sperm to penetrate the egg surroundings. Using super-resolution microscopy, we demonstrated that CD46 is relocalized during the acrosome reaction across the sperm head. This fact suggested that its role does not end with a loss of the acrosome vesicle and CD46 is probably a component of a large multiprotein complex on the sperm membrane that is responsible for sperm-egg interaction. Moreover, we showed that β1 integrins possess a similar position and behaviour as CD46 and they are present in the acrosomal area of the mouse sperm head and relocalized during AR. Thanks to STED and SIM super-resolution microscopy, we were able to distinguish a mutual position of these protein which was validated by co-localization analysis of super-resolution images. An association between CD46 and β1 integrin subunit was confirmed by proximity ligation assay. Moreover, we also defined a position of integrin α3 and α6 subunit in the sperm head and based on the obtained data they could be pairing with subunit β1, but probably occupy different sperm head compartments. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2018
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