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Transgenerational inheritance of susceptibility to diabetes-induced male subfertility
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SYSNO ASEP 0482594 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transgenerational inheritance of susceptibility to diabetes-induced male subfertility Author(s) Pavlínková, Gabriela (BTO-N) RID, ORCID
Margaryan, Hasmik (BTO-N) RID
Žatecká, Eva (BTO-N)
Valášková, Eliška (BTO-N)
Elzeinová, Fatima (BTO-N) RID
Kubátová, Alena (BTO-N) RID
Bohuslavová, Romana (BTO-N) RID
Pěknicová, Jana (BTO-N) RIDNumber of authors 8 Article number 4940 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 7, JUL 10 2017 (2017)Number of pages 14 s. Language eng - English Country GB - United Kingdom Keywords HIGH-FAT DIET ; MALE-INFERTILITY ; GENE-EXPRESSION ; SPERM PARAMETERS Subject RIV FB - Endocrinology, Diabetology, Metabolism, Nutrition OECD category Endocrinology and metabolism (including diabetes, hormones) R&D Projects NV15-30880A GA MZd - Ministry of Health (MZ) GA16-06825S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 UT WOS 000405180900013 EID SCOPUS 85034263302 DOI https://doi.org/10.1038/s41598-017-05286-0 Annotation Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality. The testicular tissue of diabetic males showed significant tissue damage, disruption of meiotic progression, and changes in the expression of genes encoding proteins important for spermiogenesis. Paternal diabetes altered sperm quality and expression pattern in the testes in offspring of two subsequent generations. Our study revealed that paternal diabetes increased susceptibility to infertility in offspring through gametic alternations. Our data also provide a mechanistic basis for transgenerational inheritance of diabetes-associated pathologies since protamines may be involved in epigenetic regulations. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2018
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