Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

Thind, A. K.; Wicker, T.; Šimková, Hana; Fossati, D.; Moullet, O.; Brabant, C.; Vrána, Jan; Doležel, Jaroslav; Krattinger, S.G.

doi:https://dx.doi.org/10.1038/nbt.3877

Number of the records: 1

Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly

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SYSNO ASEP	0482335
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Rapid cloning of genes in hexaploid wheat using cultivar-specific long-range chromosome assembly
Author(s)	Thind, A. K. (CH) Wicker, T. (CH) Šimková, Hana (UEB-Q)_{RID, ORCID} Fossati, D. (CH) Moullet, O. (CH) Brabant, C. (CH) Vrána, Jan (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Krattinger, S.G. (CH)
Number of authors	9
Source Title	Nature Biotechnology. - : Nature Publishing Group - ISSN 1087-0156 Roč. 35, č. 8 (2017), s. 793-796
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	adult-plant resistance ; leaf rust ; sequence capture ; genome ; arabidopsis ; virulence ; barley ; canada ; locus ; lr22a
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Plant sciences, botany
R&D Projects	LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000407125800028
DOI	10.1038/nbt.3877
Annotation	Cereal crops such as wheat and maize have large repeat-rich genomes that make cloning of individual genes challenging. Moreover, gene order and gene sequences often differ substantially between cultivars of the same crop species1-4. A major bottleneck for gene cloning in cereals is the generation of high-quality sequence information from a cultivar of interest. In order to accelerate gene cloning from any cropping line, we report ` targeted chromosome-based cloning via long-range assembly' (TACCA). TACCA combines lossless genome-complexity reduction via chromosome flow sorting with Chicago long-range linkage5 to assemble complex genomes. We applied TACCA to produce a high-quality (N50 of 9.76 Mb) de novo chromosome assembly of the wheat line CH Campala Lr22a in only 4 months. Using this assembly we cloned the broad-spectrum Lr22a leaf-rust resistance gene, using molecular marker information and ethyl methanesulfonate (EMS) mutants, and found that Lr22a encodes an intracellular immune receptor homologous to the Arabidopsis thaliana RPM1 protein.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2018

Number of the records: 1