Number of the records: 1  

Monitoring lipid production in yeast using SEM and Raman spectroscopy

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    SYSNO ASEP0481587
    Document TypeC - Proceedings Paper (int. conf.)
    R&D Document TypeConference Paper
    TitleMonitoring lipid production in yeast using SEM and Raman spectroscopy
    Author(s) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
    Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
    Samek, Ota (UPT-D) RID, ORCID, SAI
    Šiler, Martin (UPT-D) RID, ORCID, SAI
    Zemánek, Pavel (UPT-D) RID, SAI, ORCID
    Hároniková, A. (CZ)
    Marová, I. (CZ)
    Number of authors7
    Source Title13th Multinational Congress on Microscopy: Book of Abstracts. - Zagreb : Ruder Bošković Institute, Croatian Microscopy Society, 2017 / Gajović A. ; Weber I. ; Kovačević G. ; Čadež V. ; Šegota S. ; Peharec Štefanić P. ; Vidoš A. - ISBN 978-953-7941-19-2
    Pagess. 308-309
    Number of pages2 s.
    Publication formPrint - P
    ActionMultinational Congress on Microscopy /13./
    Event date24.09.2017 - 29.09.2017
    VEvent locationRovinj
    CountryHR - Croatia
    Event typeWRD
    Languageeng - English
    CountryHR - Croatia
    Keywordsyeast ; lipid bodies ; SEM ; Raman spectroscopy
    Subject RIVBH - Optics, Masers, Lasers
    OECD categoryElectrical and electronic engineering
    R&D ProjectsGA15-20645S GA ČR - Czech Science Foundation (CSF)
    LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUPT-D - RVO:68081731
    AnnotationThe main goal of our project is the development of specialized instrumentation and methodology to monitor metabolic states of microorganisms in order to optimize cultivation process for biotechnological applications. Here the attention is given to the oil-producing yeast strain Metschnikowia pulcherrima. The influence of different cultivation parameters (such as the effects of temperature regime and medium composition) on cells can be monitored. In our investigations on yeast cells scanning electron microscopy (SEM) and Raman spectroscopy techniques were used. Here, SEM uses electron beam to gain information about morphology of cells which reflects cells response on the different cultivation conditions. Consequently, Raman spectroscopy was used for the determination of lipids/oil present in the biomass. Thus, our study targets some factors which could lead to efficient industrial production of oil in selected biotechnological production. The yeast strains (Metschnikowia) were cultivated in medium containing different ratios of C/N (glycerol/yeast extract and/or ammonium sulphate) during a time period of two weeks. On the different days of cultivation, the cells were collected by centrifugation (2000 rpm, 1 min.) and analysed. SEM - the samples of yeast were cultivated as mentioned above and observed by cryo scanning electron microscopy (cryo-SEM).
    WorkplaceInstitute of Scientific Instruments
    ContactMartina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
    Year of Publishing2018
Number of the records: 1  

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