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Monitoring lipid production in yeast using SEM and Raman spectroscopy
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SYSNO ASEP 0481587 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Monitoring lipid production in yeast using SEM and Raman spectroscopy Author(s) Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Hároniková, A. (CZ)
Marová, I. (CZ)Number of authors 7 Source Title 13th Multinational Congress on Microscopy: Book of Abstracts. - Zagreb : Ruder Bošković Institute, Croatian Microscopy Society, 2017 / Gajović A. ; Weber I. ; Kovačević G. ; Čadež V. ; Šegota S. ; Peharec Štefanić P. ; Vidoš A. - ISBN 978-953-7941-19-2 Pages s. 308-309 Number of pages 2 s. Publication form Print - P Action Multinational Congress on Microscopy /13./ Event date 24.09.2017 - 29.09.2017 VEvent location Rovinj Country HR - Croatia Event type WRD Language eng - English Country HR - Croatia Keywords yeast ; lipid bodies ; SEM ; Raman spectroscopy Subject RIV BH - Optics, Masers, Lasers OECD category Electrical and electronic engineering R&D Projects GA15-20645S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 Annotation The main goal of our project is the development of specialized instrumentation and methodology to monitor metabolic states of microorganisms in order to optimize cultivation process for biotechnological applications. Here the attention is given to the oil-producing yeast strain Metschnikowia pulcherrima. The influence of different cultivation parameters (such as the effects of temperature regime and medium composition) on cells can be monitored. In our investigations on yeast cells scanning electron microscopy (SEM) and Raman spectroscopy techniques were used. Here, SEM uses electron beam to gain information about morphology of cells which reflects cells response on the different cultivation conditions. Consequently, Raman spectroscopy was used for the determination of lipids/oil present in the biomass. Thus, our study targets some factors which could lead to efficient industrial production of oil in selected biotechnological production. The yeast strains (Metschnikowia) were cultivated in medium containing different ratios of C/N (glycerol/yeast extract and/or ammonium sulphate) during a time period of two weeks. On the different days of cultivation, the cells were collected by centrifugation (2000 rpm, 1 min.) and analysed. SEM - the samples of yeast were cultivated as mentioned above and observed by cryo scanning electron microscopy (cryo-SEM). Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2018
Number of the records: 1