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Physical map of the short arm of bread wheat chromosome 3D
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SYSNO ASEP 0479190 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Physical map of the short arm of bread wheat chromosome 3D Author(s) Holušová, Kateřina (UEB-Q) ORCID
Vrána, Jan (UEB-Q) RID, ORCID
Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Balcárková, Barbora (UEB-Q)
Frenkel, Z. (IL)
Darrier, B. (FR)
Paux, E. (FR)
Cattonaro, F. (IT)
Berges, H. (FR)
Letellier, T. (FR)
Alaux, M. (FR)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Bartoš, Jan (UEB-Q) RID, ORCIDNumber of authors 14 Source Title Plant Genome. - : Wiley - ISSN 1940-3372
Roč. 10, č. 2 (2017)Number of pages 11 s. Language eng - English Country US - United States Keywords TRITICUM-AESTIVUM L. ; COMPLEX GENOMES ; INDIVIDUAL CHROMOSOMES Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects GA13-08786S GA ČR - Czech Science Foundation (CSF) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000410819500025 EID SCOPUS 85024474147 DOI https://doi.org/10.3835/plantgenome2017.03.0021 Annotation Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide. Although a reference genome sequence would represent a valuable resource for wheat improvement through genomics-assisted breeding and gene cloning, its generation has long been hampered by its allohexaploidy, high repeat content, and large size. As a part of a project coordinated by the International Wheat Genome Sequencing Consortium (IWGSC), a physical map of the short arm of wheat chromosome 3D (3DS) was prepared to facilitate reference genome assembly and positional gene cloning. It comprises 869 contigs with a cumulative length of 274.5 Mbp and represents 85.5% of the estimated chromosome arm size. Eighty-six Mbp of survey sequences from chromosome arm 3DS were assigned in silico to physical map contigs via next-generation sequencing of bacterial artificial chromosome pools, thus providing a high-density framework for physical map ordering along the chromosome arm. About 60% of the physical map was anchored in this single experiment. Finally, 1393 high-confidence genes were anchored to the physical map. Comparisons of gene space of the chromosome arm 3DS with genomes of closely related species [Brachypodium distachyon (L.) P.Beauv., rice (Oryza sativa L.), and sorghum [Sorghum bicolor (L.) Moench] and homeologous wheat chromosomes provided information about gene movement on the chromosome arm. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
Number of the records: 1