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RGB color coded images in scanning electron microscopy of biological surfaces
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SYSNO ASEP 0478391 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RGB color coded images in scanning electron microscopy of biological surfaces Author(s) Kofroňová, Olga (MBU-M) RID, ORCID
Benada, Oldřich (MBU-M) ORCID, RIDSource Title Acta virologica - ISSN 0001-723X
Roč. 61, č. 3 (2017), s. 349-352Number of pages 4 s. Language eng - English Country US - United States Keywords Biological surfaces ; Color images ; Scanning electron microscopy Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LO1509 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA16-20229S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000411298700016 EID SCOPUS 85028583162 DOI 10.4149/av_2017_315 Annotation We present here a methodological approach for the creation of color images in scanning electron microscopy by processing grayscale images taken simultaneously from at least three different detectors in a scanning electron microscope. The final color images are then produced by merging together those grayscale images in RGB color space. We show the images from non-conductive standard sample together with those obtained from real microbiological samples. The first one represents a microbial biofilm naturally grown on fiber glass filter. The other shows individual Bacillus subtilis cells from batch culture. All the image handling was done in open source image processing software ImageJ or GNU Image Manipulation Program (Gimp) or, alternatively, in proprietary AnalySis 3.2 Pro software processing suite. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2018
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