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Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta)
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SYSNO ASEP 0477524 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lipidomic analysis of two closely related strains of the microalga Parietochloris (Trebouxiophyceae, Chlorophyta) Author(s) Řezanka, Tomáš (MBU-M) ORCID
Nedbalová, Linda (BU-J) RID, ORCID
Lukavský, Jaromír (BU-J) RID, ORCID
Procházková, L. (CZ)
Sigler, Karel (MBU-M) RIDSource Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 25, JUL 2017 (2017), s. 473-482Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords Parietochloris ; Arachidonic acid ; Lipidomics Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Botany - Biotechnology ; Bionics R&D Projects GA17-00027S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 ; BU-J - RVO:67985939 UT WOS 000406305900049 EID SCOPUS 85021058937 DOI 10.1016/j.algal.2017.06.005 Annotation The analysis of ribosomal internal transcribed spacer 2 (ITS2) secondary structure and morphology of two strains of the green microalga Parietochloris (CCALA 1082 and 1084) confirmed that they represent one biological species. However, their fatty acid profiles showed slight differences, which was fully confirmed by a series of lipidomic analyses using both shotgun lipidomics and hydrophilic interaction liquid chromatography-tandem mass spectrometry with electrospray ionization. 26 classes of lipids were identified, including more than 430 molecular species of lipids. The analyses showed that the greatest differences are in three types of phospholipids -phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. These phospholipids have different spatial configurations (cylindrical, conical, and conical inverted), especially for those molecular species containing at least one arachidonic acid. Based on the lipidomic analyses we were able to distinguish the two strains. This may be advantageous in cases when routine DNA analysis shows that two strains are nearly identical. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2018
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