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Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay
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SYSNO ASEP 0477358 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Protein p53 Binding to Cisplatin-modified DNA Targets Evaluated by Modification-specific Electrochemical Immunoprecipitation Assay Author(s) Tichý, Vlastimil (BFU-R) RID
Šebest, Peter (BFU-R) ORCID
Orság, Petr (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Pivoňková, Hana (BFU-R) ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 6 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 29, č. 2 (2017), s. 319-323Number of pages 5 s. Publication form Print - P Language eng - English Country DE - Germany Keywords catalytic hydrogen evolution ; probe ; mechanism ; suggest Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects GAP206/11/1638 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000394998000002 DOI 10.1002/elan.201600480 Annotation Studies of protein interactions with chemically modified nucleic acids are of importance in various areas of biomolecular and biomedical research, including investigations of the binding of proteins important in medicine with DNA modified with drugs and diagnostic applications of modified DNAs in biosensing and bioanalysis. Chemical modification of DNA substrates with various species inside or outside specific protein binding sites can affect the protein-DNA recognition. In this paper we present a simple electrochemical immunoprecipitation technique designed for evaluation of the effects of antitumor drug cisplatin on the p53-DNA binding. The cisplatin-DNA adducts are utilized as electroactive labels allowing a facile determination of the p53-bound modified DNA. Effects observed using this technique accord with results of previous biochemical assays. This approach is potentially applicable in studies that deal with the influence of any electroactive DNA modifications on the protein-DNA binding. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2018
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