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Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions
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SYSNO ASEP 0477003 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Highly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal-Free Conditions Author(s) Lukovic, D. (ES)
Diez Lloret, A. (ES)
Stojkovic, P. (RS)
Rodríguez-Martínez, D. (ES)
Arago, M.P.P. (ES)
Rodriguez-Jiménez, F.J. (ES)
González-Rodríguez, P. (ES)
López-Barneo, J. (ES)
Syková, Eva (UEM-P) RID
Jendelová, Pavla (UEM-P) RID, ORCID
Kostic, J. (ES)
Moreno-Manzano, V. (ES)
Stojkovic, M. (ES)
Bhattacharya, S.S. (ES)
Erceg, Slaven (UEM-P) RID, ORCIDSource Title Stem Cells Translational Medicine. - : Oxford University Press - ISSN 2157-6564
Roč. 6, č. 4 (2017), s. 1217-1226Number of pages 10 s. Language eng - English Country US - United States Keywords cellular therapy ; clinical translation ; differentiation Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects GBP304/12/G069 GA ČR - Czech Science Foundation (CSF) LM2015064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 UT WOS 000398206500017 EID SCOPUS 85017545111 DOI 10.1002/sctm.16-0371 Annotation Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid body formation, which decreases efficiency and yield, and strongly limits medical applications. Here we develop a simple, animal-free protocol for neural conversion of both hESCs and hiPSCs in adherent culture conditions. A simple medium formula including insulin induces the direct conversion of >98% of hESCs and hiPSCs into expandable, transplantable, and functional neural progenitors with neural rosette characteristics. Further differentiation of neural progenitors into dopaminergic and spinal motoneurons as well as astrocytes and oligodendrocytes indicates that these neural progenitors retain responsiveness to instructive cues revealing the robust applicability of the protocol in the treatment of different neurodegenerative diseases. The fact that this protocol includes animal-free medium and human extracellular matrix components avoiding embryoid bodies makes this protocol suitable for the use in clinic. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2018
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