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Localization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction.
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SYSNO ASEP 0474846 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Localization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction. Author(s) Šimoník, Ondřej (BTO-N)
Dorosh, Andriy (BTO-N) RID
Frolíková, Michaela (BTO-N) ORCID
Děd, Lukáš (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Number of authors 5 Source Title Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR v. v. i., 2017 / Kubátová alena
S. 28-29Number of pages 2 s. Action XXIIIrd Symposium of Immunology and Biology of Reproduction Event date 18.05.2017 - 20.05.2017 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords monoclonal antibodies ; sperm-zona pellucida receptors ; ZP glycoproteins ; spermatozoa ; capacitation ; sperm surface proteins Subject RIV EC - Immunology OECD category Biochemistry and molecular biology Institutional support BTO-N - RVO:86652036 Annotation Although there has been recently made a great progress in knowledge of mammalian fertilization, the mammalian gametes’ interaction during their fusion has not been fully comprehended yet. The aim of our study is to localize Ezrin, Radixin and Moesin (ERM) proteins in sperm of different species and to perform a co-immunoprecipitation assay to determine a predicted interaction with proteins involved in sperm-egg interaction. Prior to fertilization, sperm have to undergo capacitation and acrosome reaction, among which actin polymerization and depolymerization plays an important role. ERM protein family acts as cross-linkers of actin and the plasma membrane. Our results of immuno-localization and Western blot demonstrate a presence of ezrin and other members of ERM family in mouse, boar and bull sperm. First selected molecule for co-immunoprecipitation assay was CD46 which is suspected to interact with ERM during sperm-egg fusion due to its described role in acrosome integrity transduction pathways and processes before the fusion of gametes. Other proteins, which are likely to interact with ERM, belong to the tetraspanin family. These proteins are essential for successful fertilization as major coordinators of a molecular network which consists of receptors or fusogens. Specifically, CD9 and CD81 are the key tetraspanins during the fusion process. CD81 is complementary to CD9 and their interaction with ERM throughout a family of four cell-surface immunoglobulin superfamily proteins, sharing a conserved glutamine-tryptophan-isoleucine motif and lining them with actin core is described in leukocytes. Based on our ERM immune-localization in sperm, we hypothesise that these proteins could interact with aforementioned tetraspanins and play important part during sperm-egg interaction and fusion. We further aim to characterise the interaction between ERM and fusion relevant molecules which could help to reveal unknown reasons connected to idiopathic infertility issues.
Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2018
Number of the records: 1