Number of the records: 1  

Localization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction.

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    SYSNO ASEP0474846
    Document TypeA - Abstract
    R&D Document TypeThe record was not marked in the RIV
    R&D Document TypeNení vybrán druh dokumentu
    TitleLocalization of ezrin/radixin/moesin in sperm and their possible association with molecules involved in sperm-egg interaction.
    Author(s) Šimoník, Ondřej (BTO-N)
    Dorosh, Andriy (BTO-N) RID
    Frolíková, Michaela (BTO-N) ORCID
    Děd, Lukáš (BTO-N) RID
    Dvořáková-Hortová, Kateřina (BTO-N)
    Number of authors5
    Source TitleBook of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR v. v. i., 2017 / Kubátová alena
    S. 28-29
    Number of pages2 s.
    ActionXXIIIrd Symposium of Immunology and Biology of Reproduction
    Event date18.05.2017 - 20.05.2017
    VEvent locationTřešť
    CountryCZ - Czech Republic
    Event typeWRD
    Languageeng - English
    CountryCZ - Czech Republic
    Keywordsmonoclonal antibodies ; sperm-zona pellucida receptors ; ZP glycoproteins ; spermatozoa ; capacitation ; sperm surface proteins
    Subject RIVEC - Immunology
    OECD categoryBiochemistry and molecular biology
    Institutional supportBTO-N - RVO:86652036
    AnnotationAlthough there has been recently made a great progress in knowledge of mammalian fertilization, the mammalian gametes’ interaction during their fusion has not been fully comprehended yet. The aim of our study is to localize Ezrin, Radixin and Moesin (ERM) proteins in sperm of different species and to perform a co-immunoprecipitation assay to determine a predicted interaction with proteins involved in sperm-egg interaction. Prior to fertilization, sperm have to undergo capacitation and acrosome reaction, among which actin polymerization and depolymerization plays an important role. ERM protein family acts as cross-linkers of actin and the plasma membrane. Our results of immuno-localization and Western blot demonstrate a presence of ezrin and other members of ERM family in mouse, boar and bull sperm. First selected molecule for co-immunoprecipitation assay was CD46 which is suspected to interact with ERM during sperm-egg fusion due to its described role in acrosome integrity transduction pathways and processes before the fusion of gametes. Other proteins, which are likely to interact with ERM, belong to the tetraspanin family. These proteins are essential for successful fertilization as major coordinators of a molecular network which consists of receptors or fusogens. Specifically, CD9 and CD81 are the key tetraspanins during the fusion process. CD81 is complementary to CD9 and their interaction with ERM throughout a family of four cell-surface immunoglobulin superfamily proteins, sharing a conserved glutamine-tryptophan-isoleucine motif and lining them with actin core is described in leukocytes. Based on our ERM immune-localization in sperm, we hypothesise that these proteins could interact with aforementioned tetraspanins and play important part during sperm-egg interaction and fusion. We further aim to characterise the interaction between ERM and fusion relevant molecules which could help to reveal unknown reasons connected to idiopathic infertility issues.






    WorkplaceInstitute of Biotechnology
    ContactMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Year of Publishing2018
Number of the records: 1  

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