The impact of type 1 diabetes on male reproduction. \n\n

0474842 - BTÚ 2018 CZ eng A - Abstract
Stádníková, S. - Kubátová, Alena - Macek, M. - Paulasová, P. - Štechová, K. - Pavlínková, Gabriela - Pěknicová, Jana
The impact of type 1 diabetes on male reproduction.

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Book of abstracts XXIIIrd Symposium of Immunology and Biology of Reproduction. Vestec u Prahy: Biotechnologický ústav AVČR v. v. i., 2017 - (Kubátová, a.). s. 24-24
[XXIIIrd Symposium of Immunology and Biology of Reproduction. 18.05.2017-20.05.2017, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA MZd(CZ) AZV 15-30880A
Institutional support: RVO:86652036
Keywords : diabetes mellitus type 1 * male sexual disfunction * insulin
Subject RIV: EC - Immunology

DM as a risk factor of male dysfertility has been recognized only recently. The aim of our study is to determine direct effects of type 1 diabetes (T1DM) on male reproductive function. 33 male patients with T1DM were approached so far and 27 agreed to participate in our study. Participants aged 23 - 47 years (median 33 years) and suffering from diabetes for 0 - 27 years (median 15 years) were assessed for presence of erectile dysfunction, depression and sexual quality of life via various questionaries (i.e. SQOL, IEEF-5, Beck depression inventory). They were also screened for diabetic complications and comorbidities - diabetic retinopathy, neuropathy, nephropathy, autoimmune thyroiditis, cardiovascular disease, hypertension and hyperlipidaemia. Serum levels of the hormones (estradiol, prolactin, testosteron, SHBG, LH, FSH and PSA) were obtained. Samples of semen were collected from the patients who were able to ejaculate (24/27). Routine analysis (semen volume, pH, viscosity, sperm count, motility and morphology) according to the WHO criteria was conducted immediately after collection. Samples were further processed for DNA quality analyses such as TUNEL assay, comet assay, Annexin V binding, ubiquitination and sperm quality testing by using monoclonal antibodies against acrosome proteins. We intent to connect detailed clinical data with these sensitive molecular techniques to determine influence of diabetic environment on spermatogenesis.






Permanent Link: http://hdl.handle.net/11104/0276725