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Reductively degradable poly(2-hydroxyethyl methacrylate) hydrogels with oriented porosity for tissue engineering applications
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SYSNO ASEP 0473652 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reductively degradable poly(2-hydroxyethyl methacrylate) hydrogels with oriented porosity for tissue engineering applications Author(s) Macková, Hana (UMCH-V) RID
Plichta, Zdeněk (UMCH-V)
Hlídková, Helena (UMCH-V) RID, ORCID
Sedláček, Ondřej (UMCH-V) RID, ORCID
Konefal, Rafal (UMCH-V) RID, ORCID
Sadakbayeva, Zhansaya (UMCH-V) RID
Dušková-Smrčková, Miroslava (UMCH-V) RID
Horák, Daniel (UMCH-V) RID, ORCID
Kubinová, Šárka (UEM-P) RID, ORCIDSource Title ACS Applied Materials and Interfaces. - : American Chemical Society - ISSN 1944-8244
Roč. 9, č. 12 (2017), s. 10544-10553Number of pages 10 s. Language eng - English Country US - United States Keywords poly(2-hydroxyethyl methacrylate) ; 2-methacryloyloxyethyl phosphorylcholine ; hydrogel Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Subject RIV - cooperation Institute of Experimental Medicine - Other Materials R&D Projects GA14-14961S GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 ; UEM-P - RVO:68378041 UT WOS 000398246900027 EID SCOPUS 85016489742 DOI 10.1021/acsami.7b01513 Annotation Degradable poly(2-hydroxyethyl methacrylate) hydrogels were prepared from a linear copolymer (Mw = 49 kDa) of 2-hydroxyethyl methacrylate (HEMA), 2-(acethylthio)ethyl methacrylate (ATEMA), and zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC). The deprotection of ATEMA thiol groups by triethylamine followed by their gentle oxidation with 2,2´-dithiodipyridine resulted in the formation of reductively degradable polymers with disulfide bridges. Finally, a hydrogel 3D structure with an oriented porosity was obtained by gelation of the polymer in the presence of needle-like sodium acetate crystals. The pore diameter and porosity of resulting poly(2-hydroxyethyl methacrylate-co-2-(acethylthio)ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) [P(HEMA-ATEMA-MPC)] hydrogels varied between 59 and 65 mu m and between 70 and 79.6 vol % according to Hg porosimetry, and complete degradation of these materials was reached in 86 days in 0.33 mmol solution of l-cysteine/L in phosphate buffer. The cross-linked P(HEMA-ATEMA-MPC) hydrogels were evaluated as a possible support for human mesenchymal stem cells (MSCs). No cytotoxicity was found for the un-cross-linked thiol-containing and protected P(HEMA-ATEMA-MPC) chains up to a concentration of 5 and 1 wt % in alpha-minimum essential medium, respectively. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2018
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