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G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression
- 1.0473579 - BFÚ 2018 RIV NL eng J - Journal Article
Renčiuk, Daniel - Ryneš, J. - Kejnovská, Iva - Foldynova-Trantirkova, S. - Andaeng, M. - Trantírek, L. - Vorlíčková, Michaela
G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression.
Biochimica et Biophysica Acta-Gene Regulatory Mechanisms. Roč. 1860, č. 2 (2017), s. 175-183. ISSN 1874-9399. E-ISSN 1876-4320
R&D Projects: GA ČR(CZ) GP14-33947P; GA ČR GAP205/12/0466; GA ČR(CZ) GA15-06785S
Institutional support: RVO:68081707
Keywords : linked polymorphic region * guanine quadruplexes * transcription factor
OECD category: Biochemistry and molecular biology
Impact factor: 5.179, year: 2017
The Oct4 gene codes for a transcription factor that plays a critical role in the maintenance of pluripotency in embryonic and cancer stem cells. Its expression thus has to be tightly regulated. We performed biophysical characterization of the promoter region using a combination of UV absorption, CD, and NMR spectroscopies, native PAGE and chemical probing, which was followed by functional studies involving luciferase reporter assays performed in osteosarcoma and human embryonic stem cell lines.
Permanent Link: http://hdl.handle.net/11104/0270707
Number of the records: 1