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On board a raft or boat in the retrovirus sea
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SYSNO ASEP 0471923 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title On board a raft or boat in the retrovirus sea Author(s) Svoboda, Jan (UMG-J) RID Number of authors 1 Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 113, č. 15 (2016), s. 3927-3931Number of pages 5 Language eng - English Country US - United States Keywords cell transformation ; virus integration ; virus rescue ; nonpermissiveness to virus infection Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-22207S GA ČR - Czech Science Foundation (CSF) Institutional support UMG-J - RVO:68378050 UT WOS 000373762400027 DOI 10.1073/pnas.1602317113 Annotation This article summarizes the essential steps in understanding the chicken Rous sarcoma virus (RSV) genome association with a nonpermissive rodent host cell genome. This insight was made possible by in-depth study of RSV-transformed rat XC cells, which were called virogenic because they indefinitely carry virus genetic information in the absence of any infectious virus production. However, the virus was rescued by association of XC cells with chicken fibroblasts, allowing cell fusion between both partners. This and additional studies led to the interpretation that the RSV genome gets integrated into the host cell genome as a provirus. Study of additional rodent virogenic cell lines provided evidence that the transcript of oncogene v-src can be transmitted to other retroviruses and produce cell transformation by itself. As discussed in the text, two main questions related to nonpermissiveness to retrovirus infection remain to be solved. The first is changes in the retrovirus envelope gene allowing virus entry into a nonpermissive cell. The second is the nature of the permissive cell functions required by the nonpermissive cell to ensure infectious virus production. Both lines of investigation are being pursued. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2017
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