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The colonization of Bacillus thuringiensis strains in bryophytes
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SYSNO ASEP 0471433 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The colonization of Bacillus thuringiensis strains in bryophytes Author(s) Lin, Q. (CN)
Zhu, P. (CN)
Carballar-Lejarazú, R. (US)
Gelbič, Ivan (BC-A) RID, ORCID
Guan, X. (CN)
Xu, L. (CN)
Zhang, L. (CN)Number of authors 7 Source Title Turkish Journal of Biology - ISSN 1300-0152
Roč. 41, č. 1 (2017), s. 41-48Number of pages 8 s. Language eng - English Country TR - Turkey Keywords Bacillus thuringiensis ; GFP ; plant colonization Subject RIV EE - Microbiology, Virology OECD category Microbiology Institutional support BC-A - RVO:60077344 UT WOS 000394539800005 EID SCOPUS 85013436348 DOI 10.3906/biy-1510-16 Annotation In our previous study, several Bacillus thuringiensis (Bt) strains were isolated from bryophyte samples, indicating that bryophytes could serve as Bt reservoirs in the wild. SFR13 is a wild strain isolated from the bryophyta Physcomitrium japonicum. In order to understand its ecological properties, green fluorescent protein (GFP)-labelled SFR13 (SFR13GFP) was generated to evaluate the colonization capability in bryophytes, using dynamic tracing and cell counting to observe the process and patterns of colonization. Our results showed that genetic stability, growth curve dynamics, and insecticidal crystal production were not affected by GFP expression in Bt. Fluorescence microscopy was used to track the dynamic distribution of SFR13GFP. Distribution patterns showed that SFR13GFP can establish stable and long-term colonization in leaves and stems by the 26th day after inoculation. A better understanding of how Bt colonizes plants in the wild will not only result in increased knowledge of plant-microbe interactions but will also lead to a more successful and reliable use of bacterial inoculants. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2018 Electronic address http://journals.tubitak.gov.tr/biology/issues/biy-17-41-1/biy-41-1-5-1510-16.pdf
Number of the records: 1