Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

0464048 - MBÚ 2017 RIV CN eng J - Journal Article
Kozáková, Hana - Schwarzer, Martin - Tučková, Ludmila - Šrůtková, Dagmar - Czarnowska, E. - Rosiak, I. - Hudcovic, Tomáš - Schabussova, I. - Hermanová, Petra - Zákostelská, Zuzana - Aleksandrzak-Piekarczyk, T. - Koryszewska-Baginska, A. - Tlaskalová-Hogenová, Helena - Cukrowska, B.
Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization.
Cellular & Molecular Immunology. Roč. 13, č. 2 (2016), s. 251-262. ISSN 1672-7681. E-ISSN 2042-0226
R&D Projects: GA ČR(CZ) GAP304/11/1252; GA ČR GA303/09/0449; GA MŠMT 7AMB15AT025
Institutional support: RVO:61388971
Keywords : allergic sensitization * germ-free * intestinal barrier
Subject RIV: EC - Immunology
Impact factor: 5.897, year: 2016

Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v 1 using a gnotobiotic mouse model. We showed that Lactobacillus (L.) rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 (TLR2) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species-and strain-dependent manners. Colonization of germ-free (GF) mice with a mixture of all three strains (Lmix) improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v 1 allergen showed significantly lower levels of allergen-specific IgE, IgG1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor (TGF)-beta level compared with the sensitized GF mice.
Permanent Link: http://hdl.handle.net/11104/0263351