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2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis
- 1.0463844 - ÚEB 2017 RIV US eng J - Journal Article
Eyer, L. - Vain, T. - Pařízková, Barbora - Oklešťková, Jana - Barbez, E. - Kozubíková, H. - Pospíšil, T. - Wierzbicka, R. - Kleine-Vehn, J. - Fránek, M. - Strnad, Miroslav - Robert, S. - Novák, Ondřej
2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis.
PLoS ONE. Roč. 11, č. 7 (2016), e0159269. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA14-34792S; GA MŠMT(CZ) LO1204; GA MŠMT LK21306
Institutional support: RVO:61389030
Keywords : PERFORMANCE LIQUID-CHROMATOGRAPHY * TANDEM MASS-SPECTROMETRY * 2,4-DICHLOROPHENOXYACETIC ACID
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.806, year: 2016
The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic and traceable analog to study auxin-related responses. Here we identified that not only exogenous 2,4-D but also its amide-linked metabolite 2,4-D-Glu displayed an inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate 2,4-D metabolite conversion, identity and activity, we have developed a novel purification procedure based on the combination of ion exchange and immuno-specific sorbents combined with a sensitive liquid chromatography-mass spectrometry method. In 2,4-D treated samples, 2,4-D-Glu and 2,4-D-Asp were detected at 100-fold lower concentrations compared to 2,4-D levels, showing that 2,4-D can be metabolized in the plant. Moreover, 2,4-D-Asp and 2,4-D-Glu were identified as reversible forms of 2,4-D homeostasis that can be converted to free 2,4-D. This work paves the way to new studies of auxin action in plant development.
Permanent Link: http://hdl.handle.net/11104/0262935
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