Number of the records: 1  

Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals

  1. 1.
    SYSNO ASEP0463732
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleEffect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals
    Author(s) Horák, Daniel (UMCH-V) RID, ORCID
    Beneš, Milan J. (UMCH-V) RID
    Procházková, Zuzana (UMCH-V)
    Trchová, Miroslava (UMCH-V) RID, ORCID
    Borysov, A. (UA)
    Pastukhov, A. (UA)
    Paliienko, K. (UA)
    Borisova, T. (UA)
    Source TitleColloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
    Roč. 149, 1 January (2017), s. 64-71
    Number of pages8 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordsglutamate ; cholesterol ; O-methyl-beta-cyclodextrin
    Subject RIVCD - Macromolecular Chemistry
    OECD categoryPolymer science
    R&D ProjectsGC16-01128J GA ČR - Czech Science Foundation (CSF)
    LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportUMCH-V - RVO:61389013
    UT WOS000390625200009
    EID SCOPUS84994385928
    DOI10.1016/j.colsurfb.2016.10.007
    AnnotationChanges in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution; moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol.
    WorkplaceInstitute of Macromolecular Chemistry
    ContactEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Year of Publishing2017
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.