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Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals
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SYSNO ASEP 0463732 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of O-methyl-.beta.-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals Author(s) Horák, Daniel (UMCH-V) RID, ORCID
Beneš, Milan J. (UMCH-V) RID
Procházková, Zuzana (UMCH-V)
Trchová, Miroslava (UMCH-V) RID, ORCID
Borysov, A. (UA)
Pastukhov, A. (UA)
Paliienko, K. (UA)
Borisova, T. (UA)Source Title Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
Roč. 149, 1 January (2017), s. 64-71Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords glutamate ; cholesterol ; O-methyl-beta-cyclodextrin Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GC16-01128J GA ČR - Czech Science Foundation (CSF) LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMCH-V - RVO:61389013 UT WOS 000390625200009 EID SCOPUS 84994385928 DOI 10.1016/j.colsurfb.2016.10.007 Annotation Changes in cholesterol concentration in the plasma membrane of presynaptic nerve terminals non-specifically modulate glutamate transport and homeostasis in the central nervous system. Reduction of the cholesterol content in isolated rat brain nerve terminals (synaptosomes) using cholesterol depleting agents decreases the glutamate uptake and increases the extracellular level of glutamate in nerve terminals. Extraction of cholesterol from the plasma membrane and its further removal from the synaptosomes by external magnetic field can be achieved by means of magnetic nanoparticles with immobilized cholesterol-depleting agent such as O-methyl-beta-cyclodextrin (MCD). A simple approach is developed for preparation of maghemite (gamma-Fe2O3) nanoparticles containing chemically bonded MCD. The method is based on preparation of a silanization agent containing MCD. It is synthesized by the reaction of triethoxy(3-isocyanatopropyl)silane with MCD. Base-catalyzed silanization of superparamagnetic gamma-Fe2O3 provides a relatively stable colloid product containing 48 mu mol of MCD g(-1). MCD-modified gamma-Fe2O3 nanoparticles decrease the initial rate of the uptake and accumulation of L-[C-14]glutamate and increase the extracellular L-[C-14]glutamate level in the preparation of nerve terminals. The effect of MCD-immobilized nanoparticles is the same as that of MCD solution; moreover, magnetic manipulation of the nanoparticles enables removal of bonded cholesterol. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2017
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