Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-alpha-l-rhamnosyl-beta-d-glucosidase active on flavonoids

0460161 - MBÚ 2017 RIV DE eng J - Journal Article
Neher, B.D. - Mazzaferro, L.S. - Kotík, Michael - Oyhenart, J. - Halada, Petr - Křen, Vladimír - Breccia, J.D.
Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-alpha-l-rhamnosyl-beta-d-glucosidase active on flavonoids.
Applied Microbiology and Biotechnology. Roč. 100, č. 7 (2016), s. 3061-3070. ISSN 0175-7598. E-ISSN 1432-0614
R&D Projects: GA MŠMT(CZ) LD13042; GA MŠMT(CZ) LD15085; GA MŠMT 7AMB13AR005
Institutional support: RVO:61388971
Keywords : alpha-L-rhamnosidase * beta-D-glucosidase * Inverting glycosidase
Subject RIV: CE - Biochemistry
Impact factor: 3.420, year: 2016

Bacteria represent an underexplored source of diglycosidases. Twenty-five bacterial strains from the genera Actinoplanes, Bacillus, Corynebacterium, Microbacterium, and Streptomyces were selected for their ability to grow in diglycosylated flavonoids-based media. The strains Actinoplanes missouriensis and Actinoplanes liguriae exhibited hesperidin deglycosylation activity (6-O-alpha-L-rhamnosyl-beta-D-glucosidase activity, EC 3.2.1.168), which was 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities. The diglycosidase production was confirmed in A. missouriensis by zymographic assays and NMR analysis of the released disaccharide, rutinose. The gene encoding the 6-O-alpha-L-rhamnosyl-beta-D-glucosidase was identified in the genome sequence of A. missouriensis 431(T) (GenBank accession number BAL86042.1) and functionally expressed in Escherichia coli. The recombinant protein hydrolyzed hesperidin and hesperidin methylchalcone, but not rutin, which indicates its specificity for 7-O-rutinosylated flavonoids.
Permanent Link: http://hdl.handle.net/11104/0260300