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Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography
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SYSNO ASEP 0460139 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography Author(s) Flodrová, Dana (UIACH-O)
Bobálová, Janette (UIACH-O) RID, ORCID
Laštovičková, Markéta (UIACH-O) RID, ORCIDNumber of authors 3 Source Title Cereal Research Communications - ISSN 0133-3720
Roč. 44, č. 2 (2016), s. 286-297Number of pages 12 s. Publication form Print - P Language eng - English Country HU - Hungary Keywords barley ; wheat ; glycoprotein ; mass spectrometry ; lectin chromatography Subject RIV CB - Analytical Chemistry, Separation R&D Projects GPP503/12/P395 GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000388000600010 EID SCOPUS 84971406539 DOI https://doi.org/10.1556/0806.44.2016.019 Annotation Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2017
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