Number of the records: 1  

LBP/BPI homologue in Eisenia andrei earthworms

  1. 1.
    SYSNO ASEP0460102
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleLBP/BPI homologue in Eisenia andrei earthworms
    Author(s) Škanta, František (MBU-M) RID
    Procházková, Petra (MBU-M) RID, ORCID
    Roubalová, Radka (MBU-M) RID, ORCID
    Dvořák, Jiří (MBU-M) RID, ORCID
    Bilej, Martin (MBU-M) RID, ORCID
    Source TitleDevelopmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X
    Roč. 54, č. 1 (2016), s. 1-6
    Number of pages6 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsEarthworm ; Eisenia ; LBP/BPI
    Subject RIVEC - Immunology
    R&D ProjectsED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    EE2.3.30.0003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportMBU-M - RVO:61388971
    UT WOS000364883100001
    EID SCOPUS84940417074
    DOI10.1016/j.dci.2015.08.008
    AnnotationLBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2017
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.