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LBP/BPI homologue in Eisenia andrei earthworms
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SYSNO ASEP 0460102 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title LBP/BPI homologue in Eisenia andrei earthworms Author(s) Škanta, František (MBU-M) RID
Procházková, Petra (MBU-M) RID, ORCID
Roubalová, Radka (MBU-M) RID, ORCID
Dvořák, Jiří (MBU-M) RID, ORCID
Bilej, Martin (MBU-M) RID, ORCIDSource Title Developmental and Comparative Immunology. - : Elsevier - ISSN 0145-305X
Roč. 54, č. 1 (2016), s. 1-6Number of pages 6 s. Language eng - English Country GB - United Kingdom Keywords Earthworm ; Eisenia ; LBP/BPI Subject RIV EC - Immunology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EE2.3.30.0003 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000364883100001 EID SCOPUS 84940417074 DOI 10.1016/j.dci.2015.08.008 Annotation LBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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