Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes

0453397 - BC 2016 RIV GB eng J - Journal Article
Lieskovská, Jaroslava - Páleníková, Jana - Langhansová, Helena - Chagas, A. C. - Calvo, E. - Kotsyfakis, Michalis - Kopecký, Jan
Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes.
Parasites & Vectors. Roč. 8, MAY 15 2015 (2015), s. 275. ISSN 1756-3305. E-ISSN 1756-3305
R&D Projects: GA ČR GAP302/12/2208
Institutional support: RVO:60077344
Keywords : Dendritic cells * Borrelia burgdorferi * Tick cystatin * Signalling
Subject RIV: EC - Immunology
Impact factor: 3.234, year: 2015

We determined the effect of cystatins on the production of chemokines in Borrelia-infected bone-marrow derived DC. The production of MIP-1 alpha was severely suppressed by both cystatins, while IP-10 was selectively inhibited only by Sialo L2. As TLR-2 is a major receptor activated by Borrelia spirochetes, we tested whether cystatins influence signalling pathways activated by TLR-2 ligand, lipoteichoic acid (LTA). Sialo L2 and weakly Sialo L attenuated the extracellular matrix-regulated kinase (Erk1/2) pathway. The activation of phosphatidylinositol-3 kinase (PI3K)/Akt pathway and nuclear factor-kappa B (NF-kappa B) was decreased only by Sialo L2. In response to Borrelia burgdorferi, the activation of Erk1/2 was impaired by Sialo L2. Production of IFN-beta was analysed in plasmacytoid DC exposed to Borrelia, TLR-7, and TLR-9 ligands. Sialo L, in contrast to Sialo L2, decreased the production of IFN-beta in pDC and also impaired the maturation of these cells.
Permanent Link: http://hdl.handle.net/11104/0254235