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Gene expression profiling of changes induced by maternal diabetes in the embryonic heart
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SYSNO ASEP 0453296 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gene expression profiling of changes induced by maternal diabetes in the embryonic heart Author(s) Bohuslavová, Romana (BTO-N) RID
Škvorová, Lada (BTO-N)
Čerychová, Radka (BTO-N)
Pavlínková, Gabriela (BTO-N) RID, ORCIDSource Title Reproductive Toxicology. - : Elsevier - ISSN 0890-6238
Roč. 57, NOV 2015 (2015), s. 147-156Number of pages 10 s. Language eng - English Country US - United States Keywords Diabetic embryopathy ; Vascular endothelial growth factor A (VEGF-A) ; Gene expression ; Cardiovascular defects Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/09/0117 GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000360322300017 DOI 10.1016/j.reprotox.2015.06.045 Annotation Cardiovascular defects are one of the most common congenital defects associated with maternal diabetes. Based on whole embryo gene expression microarray analysis, 11 genes were chosen for temporal expression analysis of diabetes-exposed hearts. The majority of the selected genes were deregulated in diabetes-exposed hearts compared to our controls at E13.5, E14.5, and E18.5. We showed increased hypoxia and HIF-1 alpha protein levels in diabetes-exposed hearts at E10.5, which is a critical time point for the induction of developmental defects associated with diabetic embryopathy. Additionally, we found increased cardiac Vegfa levels that might trigger developmental abnormalities associated with diabetic embryopathy. Our results show that maternal diabetes affects the temporal expression pattern of gene encoding molecules involved in heart development and tissue remodelling and that these molecules might affect heart maturation processes and thus, the final outcome of diabetic pregnancies. (C) 2015 Elsevier Inc. All rights reserved. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2016
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