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Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins
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SYSNO ASEP 0447354 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Mechanism of photoprotection in the cyanobacterial ancestor of plant antenna proteins Author(s) Staleva, H. (CZ)
Komenda, Josef (MBU-M) RID, ORCID
Shukla, Mahendra K. (MBU-M) RID
Šlouf, V. (CZ)
Kaňa, Radek (MBU-M) RID, ORCID
Polívka, Tomáš (BC-A) RID, ORCID
Sobotka, Roman (MBU-M) RID, ORCIDSource Title Nature Chemical Biology . - : Nature Publishing Group - ISSN 1552-4450
Roč. 11, č. 4 (2015), s. 287-291Number of pages 5 s. Language eng - English Country US - United States Keywords photosystem II ; fluorescence ; chlorophyll Subject RIV BO - Biophysics Subject RIV - cooperation Biology Centre (since 2006) - Biophysics R&D Projects GBP501/12/G055 GA ČR - Czech Science Foundation (CSF) GA14-13967S GA ČR - Czech Science Foundation (CSF) ED2.1.00/03.0110 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 ; BC-A - RVO:60077344 UT WOS 000351666500012 DOI https://doi.org/10.1038/nchembio.1755 Annotation Plants collect light for photosynthesis using light-harvesting complexes (LHCs)-an array of chlorophyll proteins that are able to reversibly switch from harvesting to energy-dissipation mode to prevent damage of the photosynthetic apparatus. LHC antennae as well as other members of the LHC superfamily evolved from cyanobacterial ancestors called high light-inducible proteins (Hlips). Here, we characterized a purified Hlip family member HliD isolated from the cyanobacterium Synechocystis sp. PCC 6803. We found that the HliD binds chlorophyll-a (Chl-a) and beta-carotene and exhibits an energy-dissipative conformation. Using femtosecond spectroscopy, we demonstrated that the energy dissipation is achieved via direct energy transfer from a Chl-a Q(y) state to the beta-carotene S-1 state. We did not detect any cation of beta-carotene that would accompany Chl-a quenching. These results provide proof of principle that this quenching mechanism operates in the LHC superfamily and also shed light on the photoprotective role of Hlips and the evolution of LHC antennae. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2016
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