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Roles of Proteome Dynamics and Cytokinin Signaling in Root to Hypocotyl Ratio Changes Induced by Shading Roots of Arabidopsis Seedlings
- 1.0446960 - BFÚ 2016 RIV JP eng J - Journal Article
Novak, J. - Černý, M. - Pavlů, J. - Zemánková, J. - Skalák, J. - Plačková, Lenka - Brzobohatý, Břetislav
Roles of Proteome Dynamics and Cytokinin Signaling in Root to Hypocotyl Ratio Changes Induced by Shading Roots of Arabidopsis Seedlings.
Plant and Cell Physiology. Roč. 56, č. 5 (2015), s. 1006-1018. ISSN 0032-0781. E-ISSN 1471-9053
R&D Projects: GA MŠMT(CZ) LO1204
Grant - others:GA ČR(CZ) GAP305/12/2144
Institutional support: RVO:68081707 ; RVO:61389030
Keywords : Arabidopsis thaliana * Cytokinin * Hypocotyl elongation
Subject RIV: BO - Biophysics; EF - Botanics (UEB-Q)
Impact factor: 4.319, year: 2015
In nature, root systems of most terrestrial plants are protected from light exposure by growing in a dark soil environment. Hence, in vitro cultivation in transparent Petri dishes leads to physiological perturbations, but the mechanisms underlying root-mediated light perception and responses have not been fully elucidated. Thus, we compared Arabidopsis thaliana seedling development in transparent and darkened Petri dishes at low light intensity (20 A mu mol m(-2) s(-1)), allowing us to follow (inter alia) hypocotyl elongation, which is an excellent process for studying interactions of signals involved in the regulation of growth and developmental responses. To obtain insights into molecular events underlying differences in seedling growth under these two conditions, we employed liquid chromatography-mass spectrometry (LC-MS) shotgun proteomics (available via the PRIDE deposit PXD001612). In total, we quantified the relative abundances of peptides representing 1,209 proteins detected in all sample replicates of LC-MS analyses.
Permanent Link: http://hdl.handle.net/11104/0248940
Number of the records: 1