Number of the records: 1
Identification of individual biofilm-forming bacterial cells using Raman tweezers
- 1.
SYSNO ASEP 0446098 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of individual biofilm-forming bacterial cells using Raman tweezers Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Holá, V. (CZ)
Růžička, F. (CZ)Number of authors 10 Source Title Journal of Biomedical Optics - ISSN 1083-3668
Roč. 20, č. 5 (2015), 051038:1-6Number of pages 6 s. Publication form Print - P Language eng - English Country US - United States Keywords Raman tweezers ; Staphylococcus epidermidis ; biofilm Subject RIV BH - Optics, Masers, Lasers R&D Projects GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000356241900043 EID SCOPUS 84924083195 DOI 10.1117/1.JBO.20.5.051038 Annotation A method for in vitro identification of individual bacterial cells is presented. The method is based on a combination of optical tweezers for spatial trapping of individual bacterial cells and Raman microspectroscopy for acquisition of spectral "Raman fingerprints" obtained from the trapped cell. Here, Raman spectra were taken from the biofilm-forming cells without the influence of an extracellular matrix and were compared with biofilmnegative cells. Results of principal component analyses of Raman spectra enabled us to distinguish between the two strains of Staphylococcus epidermidis. Thus, we propose that Raman tweezers can become the technique of choice for a clearer understanding of the processes involved in bacterial biofilms which constitute a highly privileged way of life for bacteria, protected from the external environment. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2016
Number of the records: 1