Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity

Turánek Knotigová, P.; Zyka, Daniel; Mašek, J.; Kovalová, Anna; Křupka, M.; Bartheldyová, E.; Kulich, P.; Koudelka, Š.; Lukáč, R.; Kauerová, Z.; Vacek, A.; Stuchlová Horynová, M.; Kozubík, Alois; Miller, A. D.; Fekete, Ladislav; Kratochvílová, Irena; Ježek, Jan; Ledvina, M.; Raška, M.; Turánek, J.

doi:https://dx.doi.org/10.1007/s11095-014-1516-y

Number of the records: 1

Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity

1.

SYSNO ASEP	0443903
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Molecular Adjuvants Based on Nonpyrogenic Lipophilic Derivatives of norAbuMDP/GMDP Formulated in Nanoliposomes: Stimulation of Innate and Adaptive Immunity
Author(s)	Turánek Knotigová, P. (CZ) Zyka, Daniel (UOCHB-X)_RID Mašek, J. (CZ) Kovalová, Anna (UOCHB-X) Křupka, M. (CZ) Bartheldyová, E. (CZ) Kulich, P. (CZ) Koudelka, Š. (CZ) Lukáč, R. (CZ) Kauerová, Z. (CZ) Vacek, A. (CZ) Stuchlová Horynová, M. (CZ) Kozubík, Alois (BFU-R)_{RID, ORCID} Miller, A. D. (GB) Fekete, Ladislav (FZU-D)_{RID, ORCID} Kratochvílová, Irena (FZU-D)_{RID, ORCID, SAI} Ježek, Jan (UOCHB-X)_RID Ledvina, M. (CZ) Raška, M. (CZ) Turánek, J. (CZ)
Number of authors	20
Source Title	Pharmaceutical Research. - : Springer - ISSN 0724-8741 Roč. 32, č. 4 (2015), s. 1186-1199
Number of pages	14 s.
Language	eng - English
Country	US - United States
Keywords	adjuvant ; bone-marrow radioprotection ; liposome ; muramyl dipeptide ; vaccine
Subject RIV	CC - Organic Chemistry
Subject RIV - cooperation	Institute of Physics - Physical ; Theoretical Chemistry Institute of Biophysics - Biophysics
R&D Projects	GAP304/10/1951 GA ČR - Czech Science Foundation (CSF)
	TA01011165 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Institutional support	UOCHB-X - RVO:61388963 ; BFU-R - RVO:68081707 ; FZU-D - RVO:68378271
UT WOS	000351169800002
EID SCOPUS	84925507869
DOI	10.1007/s11095-014-1516-y
Annotation	The aim of this work was to demonstrate an immunostimulatory and adjuvant effect of new apyrogenic lipophilic derivatives of norAbuMDP and norAbuGMDP formulated in nanoliposomes. Nanoliposomes and metallochelating nanoliposomes were prepared by lipid film hydration and extrusion methods. The structure of the liposomal formulation was studied by electron microscopy, AF microscopy, and dynamic light scattering. Sublethal and lethal gamma-irradiation mice models were used to demonstrate stimulation of innate immune system. Recombinant Hsp90 antigen (Candida albicans) bound onto metallochelating nanoliposomes was used for immunisation of mice to demonstrate adjuvant activities of tested compounds. Safety and stimulation of innate and adaptive immunity were demonstrated on rabbits and mice. The liposomal formulation of norAbuMDP/GMDP was apyrogenic in rabbit test and lacking any side effect in vivo. Recovery of bone marrow after sublethal gamma-irradiation as well as increased survival of mice after lethal irradiation was demonstrated. Enhancement of specific immune response was demonstrated for some derivatives incorporated in metallochelating nanoliposomes with recombinant Hsp90 protein antigen. Liposomal formulations of new lipophilic derivatives of norAbuMDP/GMDP proved themselves as promising adjuvants for recombinant vaccines as well as immunomodulators for stimulation of innate immunity and bone-marrow recovery after chemo/radio therapy of cancer.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2016

Number of the records: 1